产品名称
Fatty Acid Synthase Rabbit Polyclonal Antibody
别名
FASN; FAS; Fatty acid synthase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2194
Human Swissprot No.
P49327
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P49327/entry
Mouse Swissprot No.
P19096
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P19096
免疫原
The antiserum was produced against synthesized peptide derived from human Fatty Acid Synthase. AA range:1478-1527
特异性
Fatty Acid Synthase Polyclonal Antibody detects endogenous levels of Fatty Acid Synthase protein.
稀释度
IHC-p: 100-300.WB 1:500 - 1:2000. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008],
组织表达
Ubiquitous. Prominent expression in brain, lung, liver and mammary gland.
细胞定位
Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
信号通路
Fatty acid biosynthesis;Insulin_Receptor;
功能
catalytic activity:(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH.,catalytic activity:(3R)-3-hydroxypalmitoyl-[acyl-carrier-protein] = hexadec-2-enoyl-[acyl-carrier-protein] + H(2)O.,catalytic activity:Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein].,catalytic activity:Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+).,catalytic activity:Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein].,catalytic activity:Acyl-[acyl-carrier-protein] + NADP(+) = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADPH.,catalytic activity:Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].,catalytic activity:Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate.,function:Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.,miscellaneous:The relatively low beta-ketoacyl synthase activity may be attributable to the low 4'-phosphopantetheine content of the protein.,sequence caution:Several sequencing errors.,similarity:Contains 1 acyl carrier domain.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer which is arranged in a head to tail fashion.,tissue specificity:Ubiquitous. Prominent expression in brain, lung, and liver.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
