产品名称
ETAR Rabbit Polyclonal Antibody
别名
EDNRA; ETA; ETRA; Endothelin-1 receptor; Endothelin A receptor; ET-A; ETA-R; hET-AR
蛋白名称
Endothelin-1 receptor
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1909
Human Swissprot No.
P25101
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P25101/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13617
Mouse Swissprot No.
Q61614
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q61614
免疫原
The antiserum was produced against synthesized peptide derived from human EDNRA. AA range:378-427
特异性
ETAR Polyclonal Antibody detects endogenous levels of ETAR protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009],
组织表达
Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.
细胞定位
Cell membrane; Multi-pass membrane protein.
信号通路
Calcium;Neuroactive ligand-receptor interaction;Vascular smooth muscle contraction;
功能
function:Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.,similarity:Belongs to the G-protein coupled receptor 1 family.,subunit:Interacts with HDAC7 and HTATIP.,tissue specificity:Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
