BD-PC0001-Acrosin Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Acrosin Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PC0001

产品名称

Acrosin Rabbit Polyclonal Antibody

别名

ACR; ACRS; Acrosin

类别

常规抗体

基因名称

ACR

蛋白名称

Acrosin

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=49

Human Swissprot No.

P10323

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P10323/entry

Mouse Swissprot No.

P23578

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P23578

免疫原

Synthesized peptide derived from Acrosin . at AA range: 40-120

特异性

Acrosin Polyclonal Antibody detects endogenous levels of Acrosin protein.

稀释度

IHC-p: 100-300.WB 1:500 - 1:2000. ELISA: 1:5000.. IF 1:50-200

预测分子量

45kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008],

组织表达

Leukocyte,Testis,

细胞定位

acrosomal vesicle,extracellular region,nucleus,Golgi-associated vesicle,acrosomal matrix,protein complex,

功能

catalytic activity:Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.,function:Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 peptidase S1 domain.,subunit:Heavy chain (catalytic) and a light chain linked by two disulfide bonds.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western Blot analysis of various cells using Acrosin Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.