产品名称
ERK 3 Rabbit Polyclonal Antibody
别名
MAPK6; ERK3; PRKM6; Mitogen-activated protein kinase 6; MAP kinase 6; MAPK 6; Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK
蛋白名称
Mitogen-activated protein kinase 6
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5597
Human Swissprot No.
Q16659
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q16659/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=50772
Mouse Swissprot No.
Q61532
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q61532
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=58840
Rat Swissprot Link
http://www.uniprot.org/uniprot/P27704
免疫原
The antiserum was produced against synthesized peptide derived from human p97 MAPK. AA range:461-510
特异性
ERK 3 Polyclonal Antibody detects endogenous levels of ERK 3 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008],
组织表达
Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
细胞定位
Cytoplasm . Nucleus . Translocates to the cytoplasm following interaction with MAPKAPK5. .
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation.,function:Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.,PTM:Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
