产品名称
EphB1/2/3/4 Rabbit Polyclonal Antibody
别名
EPHB1; ELK; EPHT2; HEK6; NET; Ephrin type-B receptor 1; ELK; EPH tyrosine kinase 2; EPH-like kinase 6; EK6; hEK6; Neuronally-expressed EPH-related tyrosine kinase; NET; Tyrosine-protein kinase receptor EPH-2; EPHB2; DRT; EPHT3; EPTH3; ERK;
蛋白名称
Ephrin type-B receptor 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
2047/2048/2049/2050
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2047
Human Swissprot No.
P54762/P29323/P54753/P54760
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P54762/entry
Mouse Gene ID
270190/13845/13846
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=270190
免疫原
The antiserum was produced against synthesized peptide derived from human EPHB1/2/3/4. AA range:566-615
特异性
EphB1/2/3/4 Polyclonal Antibody detects endogenous levels of EphB1/2/3/4 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008],
组织表达
Preferentially expressed in brain.
细胞定位
Cell membrane ; Single-pass type I membrane protein . Early endosome membrane . Cell projection, dendrite .
功能
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. May be involved in cell-cell interactions in the nervous system.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,subunit:The ligand-activated form interacts with GRB2, GRB10 and NCK through their respective SH2 domains. The GRB10 SH2 domain binds EPHB1 through Tyr-928, while GRB2 binds residues within the catalytic domain. Interacts with EPHB6. The NCK SH2 domain binds EPHB1 through Tyr-594. Interacts with PRKCABP.,tissue specificity:Preferentially expressed in brain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
