产品名称
CDK5 (3D1) Mouse Monoclonal Antibody
别名
Cyclin-dependent kinase 5 (EC 2.7.11.22) (Cell division protein kinase 5) (Serine/threonine-protein kinase PSSALRE) (Tau protein kinase II catalytic subunit) (TPKII catalytic subunit)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.04% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/1020
Human Swissprot No.
Q00535
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q00535/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/12568
Mouse Swissprot No.
P49615
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P49615
Rat Gene Link
https://www.uniprot.org/uniprot/140908
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q03114
免疫原
Synthesized peptide derived from human CDK5
特异性
This antibody detects endogenous levels of CDK5 at Human, Mouse,Rat
稀释度
IHC-p1:50-200 ,WB 1:1000-2000
背景介绍
cyclin dependent kinase 5(CDK5) Homo sapiens This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants.
组织表达
[Isoform 1]: Ubiquitously expressed (PubMed:17009320, PubMed:19693690). Accumulates in cortical neurons (at protein level) (PubMed:17009320). ; [Isoform 2]: Expressed in the testis, skeletal muscle, colon, bone marrow and ovary.
细胞定位
[Isoform 1]: Cytoplasm . Nucleus . Cell membrane ; Peripheral membrane protein. Perikaryon. Cell projection, lamellipodium . Cell projection, growth cone . Cell junction, synapse, postsynaptic density . Cell junction, synapse . In axonal growth cone with extension to the peripheral lamellipodia (By similarity). Under neurotoxic stress and neuronal injury conditions, CDK5R (p35) is cleaved by calpain to generate CDK5R1 (p25) in response to increased intracellular calcium. The elevated level of p25, when in complex with CDK5, leads to its subcellular misallocation as well as its hyperactivation. Colocalizes with CTNND2 in the cell body of neuronal cells, and with CTNNB1 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells. Reversibly attached to the plasma membrane in an inactive form when complexed to dephosphorylated p35 or CDK5R2 (p39), p35 phosphorylation releases this attachment and activates CDK5. .; [Isoform 2]: Nucleus.
纯化
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
