产品名称
Eos Rabbit Polyclonal Antibody
别名
IKZF4; KIAA1782; ZNFN1A4; Zinc finger protein Eos; Ikaros family zinc finger protein 4
蛋白名称
Zinc finger protein Eos
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64375
Human Swissprot No.
Q9H2S9
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9H2S9/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=22781
Mouse Swissprot No.
Q8C208
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8C208
免疫原
The antiserum was produced against synthesized peptide derived from human IKZF4. AA range:471-520
特异性
Eos Polyclonal Antibody detects endogenous levels of Eos protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Members of the Ikaros (ZNFN1A1; MIM 603023) family of transcription factors, which includes Eos, are expressed in lymphocytes and are implicated in the control of lymphoid development.[supplied by OMIM, Jul 2002],
组织表达
Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K-562. Highly expressed in THP-1 and M-07e cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.
功能
domain:C-terminal zinc fingers mediate homodimerization.,domain:The N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.,function:DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems.,miscellaneous:'Eos' is a Greek word for rising sun.,similarity:Belongs to the Ikaros C2H2-type zinc-finger protein family.,similarity:Contains 6 C2H2-type zinc fingers.,subunit:Self-associates and interacts with other family members; IKZF1, IKZF2, IKZF3 AND IKZF5. Interacts with CTBP2, SPI1 and MITF.,tissue specificity:Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K562. Highly expressed in THP-1 and Mo7e-IL3 cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
