产品名称
ENX-2 Rabbit Polyclonal Antibody
别名
EZH1; KIAA0388; Histone-lysine N-methyltransferase EZH1; ENX-2; Enhancer of zeste homolog 1
蛋白名称
Histone-lysine N-methyltransferase EZH1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2145
Human Swissprot No.
Q92800
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q92800/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14055
Mouse Swissprot No.
P70351
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P70351
免疫原
The antiserum was produced against synthesized peptide derived from human EZH1. AA range:171-220
特异性
ENX-2 Polyclonal Antibody detects endogenous levels of ENX-2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
enhancer of zeste 1 polycomb repressive complex 2 subunit(EZH1) Homo sapiens EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009],
组织表达
Brain,Hippocampus,Uterus,
细胞定位
Nucleus . Colocalizes with trimethylated 'Lys-27' of histone H3.
功能
catalytic activity:S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.,function:Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.,similarity:Belongs to the histone-lysine methyltransferase family. EZ subfamily.,similarity:Contains 1 SET domain.,subcellular location:Colocalizes with trimethylated 'Lys-27' of histone H3.,subunit:Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2/EED-EZH1 is less abundant than the PRC2/EED-EZH2 complex, has weak methyltransferase activity and compacts chromatin in the absence of the methyltransferase cofactor S-adenosyl-L-methionine (SAM).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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