产品名称
Emp Rabbit Polyclonal Antibody
别名
MAEA; EMP; HLC10; PIG5; Macrophage erythroblast attacher; Cell proliferation-inducing gene 5 protein; Erythroblast macrophage protein; Human lung cancer oncogene 10 protein; HLC-10
蛋白名称
Macrophage erythroblast attacher
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10296
Human Swissprot No.
Q7L5Y9
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q7L5Y9/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=59003
Mouse Swissprot No.
Q4VC33
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q4VC33
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=298982
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q5RKJ1
免疫原
The antiserum was produced against synthesized peptide derived from human MAEA. AA range:181-230
特异性
Emp Polyclonal Antibody detects endogenous levels of Emp protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein that mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. The encoded protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014],
组织表达
Detected at macrophage membranes (at protein level). Ubiquitous.
细胞定位
Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix . Cell membrane . Cytoplasm, cytoskeleton . Detected in a nuclear, speckled-like pattern (PubMed:16510120). Localized with condensed chromatin at prophase; Detected in nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis (PubMed:16510120). Present in cytoplasm, nuclear matrix and at the cell surface in macrophages; predominantly nuclear in immature macrophages and predominantly detected at the cell surface in mature macrophages. Colocalizes with F-actin in macrophages (By similarity). .
功能
developmental stage:Localized with condensed chromatin at prophase; Detected in nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis.,function:Play a role in erythroblast enucleation and in the development of the mature macrophages. Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence of MAEA at cell surface of mature macrophages; This MAEA-mediated contact inhibits erythroid cells apoptosis. Participates to erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events.,similarity:Contains 1 CTLH domain.,similarity:Contains 1 LisH domain.,subcellular location:Localized as nuclear speckled-like pattern.,subunit:Form a complex with F-actin.,tissue specificity:Ubiquitous.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
