产品名称
eIF5B Rabbit Polyclonal Antibody
别名
EIF5B; IF2; KIAA0741; Eukaryotic translation initiation factor 5B; eIF-5B; Translation initiation factor IF-2
蛋白名称
Eukaryotic translation initiation factor 5B
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9669
Human Swissprot No.
O60841
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O60841/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=226982
Mouse Swissprot No.
Q05D44
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q05D44
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=308306
Rat Swissprot Link
http://www.uniprot.org/uniprot/B2GUV7
免疫原
The antiserum was produced against synthesized peptide derived from human EIF5B. AA range:1051-1100
特异性
eIF5B Polyclonal Antibody detects endogenous levels of eIF5B protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008],
组织表达
Brain,Cervix carcinoma,Epithelium,Hepatocyte,Liver,Lymph,Pl
功能
function:Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.,similarity:Belongs to the IF-2 family.,subunit:Interacts with ANXA5 in a calcium and phospholipid-dependent manner.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
