BD-PT1480-Eg5 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Eg5 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT1480

产品名称

Eg5 Rabbit Polyclonal Antibody

别名

KIF11; EG5; KNSL1; TRIP5; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5

类别

常规抗体

基因名称

KIF11

蛋白名称

Kinesin-like protein KIF11

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3832

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3832

Human Swissprot No.

P52732

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P52732/entry

Mouse Swissprot No.

Q6P9P6

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q6P9P6

免疫原

The antiserum was produced against synthesized peptide derived from human KIF11/Eg5. AA range:892-941

特异性

Eg5 Polyclonal Antibody detects endogenous levels of Eg5 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

119kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

kinesin family member 11(KIF11) Homo sapiens This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq, Jul 2008],

组织表达

Brain,Epithelium,Lung,

细胞定位

Cytoplasm . Cytoplasm, cytoskeleton, spindle pole .

功能

function:Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.,PTM:Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the kinesin-like protein family. BimC subfamily.,similarity:Contains 1 kinesin-motor domain.,subunit:Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using KIF11/Eg5 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using KIF11/Eg5 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat, HepG2, and COS cells, using KIF11/Eg5 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Eg5 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of COLO205 cells using Eg5 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.