产品名称
EF-2 Rabbit Polyclonal Antibody
别名
EEF2; EF2; Elongation factor 2; EF-2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1938
Human Swissprot No.
P13639
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P13639/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13629
Mouse Swissprot No.
P58252
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P58252
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29565
Rat Swissprot Link
http://www.uniprot.org/uniprot/P05197
免疫原
The antiserum was produced against synthesized peptide derived from human eEF2. AA range:31-80
特异性
EF-2 Polyclonal Antibody detects endogenous levels of EF-2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008],
组织表达
Brain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph
细胞定位
Cytoplasm . Nucleus . Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product. .
功能
function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.,PTM:Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.,PTM:Phosphorylation by EF-2 kinase completely inactivates EF-2.,similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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