产品名称
Dorfin Rabbit Polyclonal Antibody
别名
RNF19A; RNF19; E3 ubiquitin-protein ligase RNF19A; Double ring-finger protein; Dorfin; RING finger protein 19A; p38
蛋白名称
E3 ubiquitin-protein ligase RNF19A
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25897
Human Swissprot No.
Q9NV58
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9NV58/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=30945
Mouse Swissprot No.
P50636
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P50636
免疫原
The antiserum was produced against synthesized peptide derived from human RNF19A. AA range:51-100
特异性
Dorfin Polyclonal Antibody detects endogenous levels of Dorfin protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the ring between ring fingers (RBR) protein family, and the encoded protein contains two RING-finger motifs and an in between RING fingers motif. This protein is an E3 ubiquitin ligase that is localized to Lewy bodies, and ubiquitylates synphilin-1, which is an interacting protein of alpha synuclein in neurons. The encoded protein may be involved in amyotrophic lateral sclerosis and Parkinson's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
组织表达
Widely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system.
细胞定位
Membrane ; Multi-pass membrane protein . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
功能
function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the RBR family. RNF19 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 2 RING-type zinc fingers.,subcellular location:Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.,subunit:Interacts with UBE2L3 and UBE2L6. Interacts with transcription factor Sp1. Interacts with VCP, CASR, SNCAIP and with some SOD1 variants which cause amyotrophic lateral sclerosis, but not with wild-type SOD1.,tissue specificity:Widely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
