产品名称
Dok-5 Rabbit Polyclonal Antibody
别名
DOK5; C20orf180; Docking protein 5; Downstream of tyrosine kinase 5; Insulin receptor substrate 6; IRS-6; IRS6
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=55816
Human Swissprot No.
Q9P104
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9P104/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=76829
Mouse Swissprot No.
Q91ZM9
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q91ZM9
免疫原
The antiserum was produced against synthesized peptide derived from human DOK5. AA range:101-150
特异性
Dok-5 Polyclonal Antibody detects endogenous levels of Dok-5 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
docking protein 5(DOK5) Homo sapiens The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. Unlike other DOK family proteins, this protein does not interact with RASGAP. This protein is up-regulated in patients with systemic sclerosis and is associated with fibrosis induced by insulin-like growth factor binding protein 5. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jun 2014],
组织表达
Highest expression in skeletal muscle, lower in brain, heart and kidney. Also detected in activated peripheral blood T-lymphocytes.
功能
domain:PTB domain mediates receptor interaction.,function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.,PTM:Phosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF.,similarity:Belongs to the DOK family. Type B subfamily.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:Interacts with phosphorylated RET. In contrast to other DOK proteins, it does not interact with RASGAP.,tissue specificity:Highest expression in skeletal muscle, lower in brain, heart and kidney. Also detected in activated peripheral blood T-lymphocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
