产品名称
Dok-4 Rabbit Polyclonal Antibody
别名
DOK4; Docking protein 4; Downstream of tyrosine kinase 4; Insulin receptor substrate 5; IRS-5; IRS5
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=55715
Human Swissprot No.
Q8TEW6
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q8TEW6/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=114255
Mouse Swissprot No.
Q99KE3
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q99KE3
免疫原
The antiserum was produced against synthesized peptide derived from human DOK4. AA range:11-60
特异性
Dok-4 Polyclonal Antibody detects endogenous levels of Dok-4 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
domain:PTB domain mediates receptor interaction.,function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.,PTM:Phosphorylated on tyrosine residues in response to insulin, IGF1 or RET stimulation.,similarity:Belongs to the DOK family. Type B subfamily.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:Interacts with RET and TEK/TIE2. Interaction with RET is mediated through the PTB domain and requires phosphorylation of RET 'Tyr-1062'.,tissue specificity:Widely expressed. High expression in skeletal muscle, heart, kidney and liver. Weaker expression in spleen, lung and small intestine, brain, heart and. Expressed in both resting and activated peripheral blood T-cells.,
组织表达
Widely expressed. High expression in skeletal muscle, heart, kidney and liver. Weaker expression in spleen, lung and small intestine, brain, heart and. Expressed in both resting and activated peripheral blood T-cells.
功能
domain:PTB domain mediates receptor interaction.,function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.,PTM:Phosphorylated on tyrosine residues in response to insulin, IGF1 or RET stimulation.,similarity:Belongs to the DOK family. Type B subfamily.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:Interacts with RET and TEK/TIE2. Interaction with RET is mediated through the PTB domain and requires phosphorylation of RET 'Tyr-1062'.,tissue specificity:Widely expressed. High expression in skeletal muscle, heart, kidney and liver. Weaker expression in spleen, lung and small intestine, brain, heart and. Expressed in both resting and activated peripheral blood T-cells.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
