产品名称
DOCK 180 Rabbit Polyclonal Antibody
别名
DOCK1; Dedicator of cytokinesis protein 1; 180 kDa protein downstream of CRK; DOCK180
蛋白名称
Dedicator of cytokinesis protein 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1793
Human Swissprot No.
Q14185
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q14185/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=330662
Mouse Swissprot No.
Q8BUR4
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BUR4
免疫原
The antiserum was produced against synthesized peptide derived from human DOCK1. AA range:1661-1710
特异性
DOCK 180 Polyclonal Antibody detects endogenous levels of DOCK 180 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014],
组织表达
Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
细胞定位
Cytoplasm . Membrane . Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-trisphosphate. .
信号通路
Focal adhesion;Regulates Actin and Cytoskeleton;
功能
domain:The DHR-2 domain is necessary and sufficient for the GEF activity.,function:Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.,similarity:Belongs to the DOCK family.,similarity:Contains 1 DHR-1 (CZH-1) domain.,similarity:Contains 1 DHR-2 (CZH-2) domain.,similarity:Contains 1 SH3 domain.,subcellular location:Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.,subunit:Interacts with the SH3 domains of CRK and NCK2 via multiple sites. Interacts with nucleotide-free RAC1 via its DHR-2 domain. Interacts with ELMO1, ELMO2 and probably ELMO3 via its SH3 domain. Interacts with RAC1 and BAI1.,tissue specificity:Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
