BD-PT1377

DNA pol ι Rabbit Polyclonal Antibody

POLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B

常规抗体

POLI

DNA polymerase iota

WB

Human,Rat,Mouse

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

11201

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11201

Q9UNA4

http://www.uniprot.org/uniprotkb/Q9UNA4/entry

Q6R3M4

http://www.uniprot.org/uniprot/Q6R3M4

The antiserum was produced against synthesized peptide derived from human POLI. AA range:641-690

DNA pol ι Polyclonal Antibody detects endogenous levels of DNA pol ι protein.

WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

85kD

-20°C/1 year

Polyclonal, Rabbit,IgG

catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Magnesium.,domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.,function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.,similarity:Belongs to the DNA polymerase type-Y family.,similarity:Contains 1 umuC domain.,subcellular location:Accumulates at replication forks after DNA damage.,subunit:Binds REV1L (By similarity). Binds POLH.,tissue specificity:Ubiquitous. Highly expressed in testis.,

Ubiquitous. Highly expressed in testis.

Nucleus . Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage. .

catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Magnesium.,domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.,function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.,similarity:Belongs to the DNA polymerase type-Y family.,similarity:Contains 1 umuC domain.,subcellular location:Accumulates at replication forks after DNA damage.,subunit:Binds REV1L (By similarity). Binds POLH.,tissue specificity:Ubiquitous. Highly expressed in testis.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.