产品名称
DNA pol δ cat Rabbit Polyclonal Antibody
别名
POLD1; POLD; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125
蛋白名称
DNA polymerase delta catalytic subunit
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5424
Human Swissprot No.
P28340
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P28340/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18971
Mouse Swissprot No.
P52431
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P52431
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=59294
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54747
免疫原
The antiserum was produced against synthesized peptide derived from human POLD1. AA range:1051-1100
特异性
DNA pol δ cat Polyclonal Antibody detects endogenous levels of DNA pol δ cat protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012],
组织表达
Widely expressed, with high levels of expression in heart and lung.
细胞定位
Nucleus . Colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). After UV irradiation, recruited to DNA damage sites within 2 hours, independently on the cell cycle phase, nor on PCNA ubiquitination. This recruitment requires POLD3, PCNA and RFC1-replication factor C complex (PubMed:20227374, PubMed:22801543). .
信号通路
Purine metabolism;Pyrimidine metabolism;DNA replication;Base excision repair;Nucleotide excision repair;Mismatch repair;Homologous recombination;
功能
catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,similarity:Belongs to the DNA polymerase type-B family.,subunit:Heterotetramer composed of subunits of 125 kDa, 50 kDa, 66 kDa and 12 kDa. The 125 kDa subunit contains the polymerase active site and most likely the active site for the 3'-5' exonuclease activity. Interacts with WRNIP1. Interacts with POLD4 and PCNA.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
