产品名称
DNA pol β Rabbit Polyclonal Antibody
别名
POLB; DNA polymerase beta
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5423
Human Swissprot No.
P06746
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P06746/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18970
Mouse Swissprot No.
Q8K409
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8K409
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29240
Rat Swissprot Link
http://www.uniprot.org/uniprot/P06766
免疫原
The antiserum was produced against synthesized peptide derived from human DNA Polymerase beta. AA range:286-335
特异性
DNA pol β Polyclonal Antibody detects endogenous levels of DNA pol β protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011],
细胞定位
Nucleus. Cytoplasm. Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.
信号通路
Base excision repair;
功能
catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Binds 2 magnesium ions per subunit.,domain:Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.,function:Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.,PTM:Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.,similarity:Belongs to the DNA polymerase type-X family.,subunit:Monomer.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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