产品名称
DNA pol α Rabbit Polyclonal Antibody
别名
POLA1; POLA; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180
蛋白名称
DNA polymerase alpha catalytic subunit
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5422
Human Swissprot No.
P09884
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P09884/entry
Mouse Swissprot No.
P33609
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P33609
免疫原
The antiserum was produced against synthesized peptide derived from human DNA Polymerase alpha. AA range:81-130
特异性
DNA pol α Polyclonal Antibody detects endogenous levels of DNA pol α protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010],
细胞定位
Nucleus . Cytoplasm, cytosol . In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern. .
信号通路
Purine metabolism;Pyrimidine metabolism;DNA replication;
功能
catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Polymerase alpha in a complex with DNA primase is a replicative polymerase.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,PTM:A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.,similarity:Belongs to the DNA polymerase type-B family.,subunit:Interacts with SV40 Large T antigen; this interaction allows viral DNA replication.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
