产品名称
DNA Ligase III Rabbit Polyclonal Antibody
别名
LIG3; DNA ligase 3; DNA ligase III; Polydeoxyribonucleotide synthase [ATP] 3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3980
Human Swissprot No.
P49916
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P49916/entry
Mouse Swissprot No.
P97386
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P97386
免疫原
Synthesized peptide derived from DNA Ligase III . at AA range: 110-190
特异性
DNA Ligase III Polyclonal Antibody detects endogenous levels of DNA Ligase III protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],
组织表达
Testis, thymus, prostate and heart.
细胞定位
[Isoform 1]: Mitochondrion . Contains an N-terminal mitochondrial transit peptide. .; [Isoform 2]: Mitochondrion . Contains an N-terminal mitochondrial transit peptide. .; [Isoform 3]: Nucleus . Lacks the N-terminal mitochondrial transit peptide. .; [Isoform 4]: Nucleus . Lacks the N-terminal mitochondrial transit peptide. .
信号通路
Base excision repair;
功能
catalytic activity:ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).,cofactor:Magnesium.,function:Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.,online information:DNA ligase entry,similarity:Belongs to the ATP-dependent DNA ligase family.,similarity:Contains 1 BRCT domain.,similarity:Contains 1 PARP-type zinc finger.,tissue specificity:Testis, thymus, prostate and heart.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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