产品名称
DGK-κ Rabbit Polyclonal Antibody
别名
DGKK; Diacylglycerol kinase kappa; DAG kinase kappa; 142 kDa diacylglycerol kinase; Diglyceride kinase kappa; DGK-kappa
蛋白名称
Diacylglycerol kinase kappa
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=139189
Human Swissprot No.
Q5KSL6
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q5KSL6/entry
免疫原
The antiserum was produced against synthesized peptide derived from human DGKK. AA range:1221-1270
特异性
DGK-κ Polyclonal Antibody detects endogenous levels of DGK-κ protein.
稀释度
WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is an enzyme that phosphorylates diacylglycerol, converting it to phosphatidic acid. The encoded protein is a membrane protein and is inhibited by hydrogen peroxide. Variations in this gene have been associated with hypospadias. [provided by RefSeq, Mar 2011],
组织表达
Expressed in testis, and to a lesser extent in placenta.
细胞定位
Cell membrane ; Peripheral membrane protein .
功能
catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,enzyme regulation:Inhibited in response to H(2)O(2).,function:Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).,PTM:Phosphorylated at Tyr-78 by some member of the SRC family in response to H(2)O(2).,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 1 PH domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Does not form homooligomers.,tissue specificity:Expressed in testis, and to a lesser extent in placenta.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
