产品名称
DGK-θ Rabbit Polyclonal Antibody
别名
DGKQ; DAGK4; Diacylglycerol kinase theta; DAG kinase theta; Diglyceride kinase theta; DGK-theta
蛋白名称
Diacylglycerol kinase theta
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1609
Human Swissprot No.
P52824
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P52824/entry
Mouse Swissprot No.
Q6P5E8
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q6P5E8
免疫原
The antiserum was produced against synthesized peptide derived from human DGKQ. AA range:691-740
特异性
DGK-θ Polyclonal Antibody detects endogenous levels of DGK-θ protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008],
细胞定位
Cytoplasm . Cytoplasm, cytosol . Cell membrane . Cell junction, synapse . Cytoplasm, cytoskeleton . Nucleus . Nucleus speckle . Nucleus matrix . Translocates to the plasma membrane in response to steroid hormone receptor stimulation (PubMed:15632189). Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH (PubMed:15632189). Translocates to the nucleus in response to thrombin stimulation (Probable). Association with the nuclear matrix is regulated by nerve growth factor (By similarity). .
信号通路
Glycerolipid metabolism;Glycerophospholipid metabolism;Phosphatidylinositol signaling system;
功能
catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 1 Ras-associating domain.,similarity:Contains 3 phorbol-ester/DAG-type zinc fingers.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
