产品名称
DGK-ε Rabbit Polyclonal Antibody
别名
DGKE; DAGK5; Diacylglycerol kinase epsilon; DAG kinase epsilon; Diglyceride kinase epsilon; DGK-epsilon
蛋白名称
Diacylglycerol kinase epsilon
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8526
Human Swissprot No.
P52429
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P52429/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56077
Mouse Swissprot No.
Q9R1C6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9R1C6
免疫原
The antiserum was produced against synthesized peptide derived from human DGKE. AA range:161-210
特异性
DGK-ε Polyclonal Antibody detects endogenous levels of DGK-ε protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008],
组织表达
Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).
细胞定位
Membrane ; Single-pass membrane protein . Cytoplasm .
信号通路
Glycerolipid metabolism;Glycerophospholipid metabolism;Phosphatidylinositol signaling system;
功能
catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,function:Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,tissue specificity:Expressed predominantly in testis.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
