产品名称
DGK-β Rabbit Polyclonal Antibody
别名
DGKB; DAGK2; KIAA0718; Diacylglycerol kinase beta; DAG kinase beta; 90 kDa diacylglycerol kinase; Diglyceride kinase beta; DGK-beta
蛋白名称
Diacylglycerol kinase beta
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1607
Human Swissprot No.
Q9Y6T7
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9Y6T7/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=217480
Mouse Swissprot No.
Q6NS52
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q6NS52
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54248
Rat Swissprot Link
http://www.uniprot.org/uniprot/P49621
免疫原
The antiserum was produced against synthesized peptide derived from human DGKB. AA range:657-706
特异性
DGK-β Polyclonal Antibody detects endogenous levels of DGK-β protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008],
组织表达
[Isoform 1]: Specifically expressed in brain but also detected in uterus (PubMed:11719522). In adult brain, expressed in the amygdala, caudate nucleus, and hippocampus (PubMed:11719522). ; [Isoform 2]: More ubiquitously expressed but at lower level compared to isoform 1.
细胞定位
Cell junction, synapse, postsynaptic cell membrane ; Peripheral membrane protein . Cell membrane ; Peripheral membrane protein . Cytoplasm . Translocation to the plasma membrane is induced by phorbol esters. .; [Isoform 2]: Cytoplasm .
信号通路
Glycerolipid metabolism;Glycerophospholipid metabolism;Phosphatidylinositol signaling system;
功能
catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,enzyme regulation:Stimulated by phosphatidylserine.,function:Exhibits high phosphorylation activity for long-chain diacylglycerols.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 EF-hand domains.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
