BD-PT1321-DEF-3 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

DEF-3 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT1321

产品名称

DEF-3 Rabbit Polyclonal Antibody

别名

RBM6; DEF3; RNA-binding protein 6; Lung cancer antigen NY-LU-12; Protein G16; RNA-binding motif protein 6; RNA-binding protein DEF-3

类别

常规抗体

基因名称

RBM6

蛋白名称

RNA-binding protein 6

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

10180

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10180

Human Swissprot No.

P78332

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P78332/entry

免疫原

The antiserum was produced against synthesized peptide derived from human RBM6. AA range:991-1040

特异性

DEF-3 Polyclonal Antibody detects endogenous levels of DEF-3 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

150kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

disease:Defects in RBM6 are found in non-small cell lung cancer (NSCLC) cell lines.,function:Specifically binds poly(G) RNA homopolymers in vitro.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 G-patch domain.,similarity:Contains 1 RRM (RNA recognition motif) domain.,tissue specificity:Ubiquitous in adults.,

组织表达

Ubiquitous in adults.

细胞定位

Nucleus .

功能

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of COS7 cells, using RBM6 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using RBM6 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HUVEC cells, using RBM6 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.