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DDX55 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT1317
产品名称
DDX55 Rabbit Polyclonal Antibody
别名
DDX55; KIAA1595; ATP-dependent RNA helicase DDX55; DEAD box protein 55
类别
常规抗体
基因名称
DDX55
蛋白名称
ATP-dependent RNA helicase DDX55
推荐应用
WB
反应种属
Human,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
57696
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=57696
Human Swissprot No.
Q8NHQ9
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q8NHQ9/entry
Mouse Gene ID
67848
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=67848
Mouse Swissprot No.
Q6ZPL9
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q6ZPL9
免疫原
The antiserum was produced against synthesized peptide derived from human DDX55. AA range:91-140
特异性
DDX55 Polyclonal Antibody detects endogenous levels of DDX55 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
预测分子量
68kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of protein family containing a characteristic Asp-Glu-Ala-Asp (DEAD) motif. These proteins are putative RNA helicases, and may be involved in a range of nuclear processes including translational initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Multiple alternatively spliced transcript variants have been found for this gene. Pseudogenes have been identified on chromosomes 1 and 12. [provided by RefSeq, Feb 2016],
组织表达
Eye,Stomach,Testis,
细胞定位
membrane,
功能
domain:The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.,function:Probable ATP-binding RNA helicase.,similarity:Belongs to the DEAD box helicase family.,similarity:Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from HeLa cells, using DDX55 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using DDX55 Polyclonal Antibody diluted at 1:500

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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