产品名称
DD2 Rabbit Polyclonal Antibody
别名
AKR1C2; DDH2; Aldo-keto reductase family 1 member C2; 3-alpha-HSD3; Chlordecone reductase homolog HAKRD; Dihydrodiol dehydrogenase 2; DD-2; DD2; Dihydrodiol dehydrogenase/bile acid-binding protein; DD/BABP; Trans-1; 2-dihydrobenzene-1,2-diol
蛋白名称
Aldo-keto reductase family 1 member C2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1646
Human Swissprot No.
P52895
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P52895/entry
免疫原
The antiserum was produced against synthesized peptide derived from human AKR1C2. AA range:21-70
特异性
DD2 Polyclonal Antibody detects endogenous levels of DD2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011],
组织表达
Expressed in fetal testes. Expressed in fetal and adult adrenal glands.
细胞定位
Cytoplasm, cytosol .
信号通路
Steroid hormone biosynthesis;Metabolism of xenobiotics by cytochrome P450;
功能
catalytic activity:Androsterone + NAD(P)(+) = 5-alpha-androstane-3,17-dione + NAD(P)H.,catalytic activity:Trans-1,2-dihydrobenzene-1,2-diol + NADP(+) = catechol + NADPH.,enzyme regulation:Inhibited by hexestrol with an IC(50) of 2.8 uM, 1,10-phenanthroline with an IC(50) of 2100 uM, 1,7-phenanthroline with an IC(50) of 1500 uM, flufenamic acid with an IC(50) of 0.9 uM, indomethacin with an IC(50) of 75 uM, ibuprofen with an IC(50) of 6.9 uM, lithocholic acid with an IC(50) of 0.07 uM, ursodeoxycholic acid with an IC(50) of 0.08 uM and chenodeoxycholic acid with an IC(50) of 0.13 uM.,function:Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability.,similarity:Belongs to the aldo/keto reductase family.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
