产品名称
DBH Rabbit Polyclonal Antibody
别名
DBH; Dopamine beta-hydroxylase; Dopamine beta-monooxygenase
蛋白名称
Dopamine beta-hydroxylase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1621
Human Swissprot No.
P09172
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P09172/entry
Mouse Swissprot No.
Q64237
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q64237
免疫原
The antiserum was produced against synthesized peptide derived from human DBH. AA range:401-450
特异性
DBH Polyclonal Antibody detects endogenous levels of DBH protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008],
细胞定位
[Soluble dopamine beta-hydroxylase]: Cytoplasmic vesicle, secretory vesicle lumen . Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen . Secreted .; Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass type II membrane protein . Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane ; Single-pass type II membrane protein .
信号通路
Tyrosine metabolism;
功能
catalytic activity:3,4-dihydroxyphenethylamine + ascorbate + O(2) = noradrenaline + dehydroascorbate + H(2)O.,cofactor:Binds 1 PQQ per subunit.,cofactor:Binds 2 copper ions per subunit.,disease:Defects in DBH are the cause of DBH deficiency [MIM:223360]; also called norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.,function:Conversion of dopamine to noradrenaline.,induction:Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids.,online information:Dopamine beta hydroxylase entry,pathway:Catecholamine biosynthesis; norepinephrine biosynthesis; norepinephrine from dopamine: step 1/1.,polymorphism:There are two main alleles of DBH: DBH-A with Ala-318 and DBH-B with Ser-318.,similarity:Belongs to the copper type II ascorbate-dependent monooxygenase family.,similarity:Contains 1 DOMON domain.,subunit:Homotetramer composed of two non-covalently bound disulfide-linked dimers.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
