BD-PT1291-DARPP-32 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

DARPP-32 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT1291

产品名称

DARPP-32 Rabbit Polyclonal Antibody

别名

PPP1R1B; DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein

类别

常规抗体

基因名称

PPP1R1B

蛋白名称

Protein phosphatase 1 regulatory subunit 1B

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

84152

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84152

Human Swissprot No.

Q9UD71

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q9UD71/entry

Mouse Gene ID

19049

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19049

Mouse Swissprot No.

Q60829

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q60829

Rat Gene ID

360616

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=360616

Rat Swissprot No.

Q6J4I0

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q6J4I0

免疫原

The antiserum was produced against synthesized peptide derived from human DARPP-32. AA range:41-90

特异性

DARPP-32 Polyclonal Antibody detects endogenous levels of DARPP-32 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

预测分子量

32kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],

组织表达

Adipose tissue,Brain,Cerebellum,Colon,Ovary,

细胞定位

Cytoplasm.

功能

function:Inhibitor of protein-phosphatase 1.,PTM:Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.,PTM:Phosphorylation of Thr-34 is required for activity.,similarity:Belongs to the protein phosphatase inhibitor 1 family.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DARPP-32 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 30‘, using DARPP-32 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using DARPP-32 Polyclonal Antibody diluted at 1：500

Western Blot analysis of COLO205 cells using DARPP-32 Polyclonal Antibody diluted at 1：500

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.