产品名称
DAPK1 Rabbit Polyclonal Antibody
别名
DAPK1; DAPK; Death-associated protein kinase 1; DAP kinase 1
蛋白名称
Death-associated protein kinase 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1612
Human Swissprot No.
P53355
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P53355/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=69635
Mouse Swissprot No.
Q80YE7
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q80YE7
免疫原
The antiserum was produced against synthesized peptide derived from human DAPK1. AA range:274-323
特异性
DAPK1 Polyclonal Antibody detects endogenous levels of DAPK1 protein.
稀释度
WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
组织表达
Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.
细胞定位
[Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton.
信号通路
Pathways in cancer;Bladder cancer;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-308.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,induction:Up-regulated following treatment with interferon-gamma.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 10 ANK repeats.,subcellular location:Colocalizes with the actin filament system.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
