BD-PT1269-Cytokeratin 19 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Cytokeratin 19 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT1269

产品名称

Cytokeratin 19 Rabbit Polyclonal Antibody

别名

KRT19; Keratin; type I cytoskeletal 19; Cytokeratin-19; CK-19; Keratin-19; K19

类别

常规抗体

基因名称

KRT19

蛋白名称

Keratin type I cytoskeletal 19

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3880

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3880

Human Swissprot No.

P08727

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P08727/entry

Mouse Gene ID

16669

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16669

Mouse Swissprot No.

P19001

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P19001

Rat Gene ID

360626

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=360626

Rat Swissprot No.

Q63279

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q63279

免疫原

The antiserum was produced against synthesized peptide derived from human Keratin 19. AA range:231-280

特异性

Cytokeratin 19 Polyclonal Antibody detects endogenous levels of Cytokeratin 19 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

预测分子量

44kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008],

组织表达

Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.

细胞定位

intermediate filament,plasma membrane,dystrophin-associated glycoprotein complex,Z disc,sarcolemma,costamere,extracellular exosome,cell periphery,terminal web,

功能

developmental stage:Present in hair follicles at all stages of development.,domain:This keratin differs from all other IF proteins in lacking the C-terminal tail domain.,function:Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. Interacts with PNN and the actin-binding domain of DMD. Interacts with HCV core protein.,tissue specificity:Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Keratin 19 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from LOVO cells, using Keratin 19 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of human-liver tissue. 1,Cytokeratin 19 Polyclonal Antibody（red） was diluted at 1:200（4°,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-lung tissue. 1,Cytokeratin 19 Polyclonal Antibody（red） was diluted at 1:200（4°,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,CytokeRatin 19 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,CytokeRatin 19 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,CytokeRatin 19 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,CytokeRatin 19 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,CytokeRatin 19 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,CytokeRatin 19 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of various cells using Cytokeratin 19 Polyclonal Antibody diluted at 1：1000

Western Blot analysis of HY926 cells using Cytokeratin 19 Polyclonal Antibody diluted at 1：1000