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Cystatin A Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT1249
产品名称
Cystatin A Rabbit Polyclonal Antibody
别名
CSTA; STF1; STFA; Cystatin-A; Cystatin-AS; Stefin-A
类别
常规抗体
基因名称
CSTA
蛋白名称
Cystatin-A
推荐应用
WB
反应种属
Human,Rat,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
1475
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1475
Human Swissprot No.
P01040
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P01040/entry
Mouse Swissprot No.
P56567
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P56567
免疫原
The antiserum was produced against synthesized peptide derived from human Stefin A. AA range:49-98
特异性
Cystatin A Polyclonal Antibody detects endogenous levels of Cystatin A protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
预测分子量
11kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008],
组织表达
Expressed in the skin throughout the epidermis.
细胞定位
Cytoplasm .
功能
function:This is an intracellular thiol proteinase inhibitor.,similarity:Belongs to the cystatin family.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using Stefin A Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Stefin A Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HT29 cells, using Stefin A Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Cystatin A Polyclonal Antibody

Western Blot analysis of HT29 cells using Cystatin A Polyclonal Antibody

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