产品名称
CYP8B1 Rabbit Polyclonal Antibody
别名
CYP8B1; CYP12; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase
蛋白名称
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1582
Human Swissprot No.
Q9UNU6
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9UNU6/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13124
Mouse Swissprot No.
O88962
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O88962
免疫原
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 8B1. AA range:371-420
特异性
CYP8B1 Polyclonal Antibody detects endogenous levels of CYP8B1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008],
细胞定位
Endoplasmic reticulum membrane ; Single-pass membrane protein . Microsome membrane ; Single-pass membrane protein .
信号通路
Primary bile acid biosynthesis;PPAR;
功能
catalytic activity:7-alpha-hydroxycholest-4-en-3-one + NADPH + O(2) = 7-alpha,12-alpha-dihydroxycholest-4-en-3-one + NADP(+) + H(2)O.,cofactor:Heme group.,function:Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Liver.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
