产品名称
CYP2R1 Rabbit Polyclonal Antibody
别名
CYP2R1; Vitamin D 25-hydroxylase; Cytochrome P450 2R1
蛋白名称
Vitamin D 25-hydroxylase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=120227
Human Swissprot No.
Q6VVX0
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q6VVX0/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=244209
Mouse Swissprot No.
Q6VVW9
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q6VVW9
免疫原
The antiserum was produced against synthesized peptide derived from human CYP2R1. AA range:251-300
特异性
CYP2R1 Polyclonal Antibody detects endogenous levels of CYP2R1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in this gene has been associated with selective 25-hydroxyvitamin D deficiency. [provided by RefSeq, Jul 2008],
细胞定位
Endoplasmic reticulum membrane ; Peripheral membrane protein. Microsome membrane ; Peripheral membrane protein.
功能
catalytic activity:5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + NADPH + O(2) = (25R)-5-beta-cholestane-3-alpha,7-alpha,12-alpha,26-tetraol + NADP(+) + H(2)O.,cofactor:Heme group.,disease:Defects in CYP2R1 are a cause of 25-hydroxyvitamin D(3) deficiency (25HOD3D)[MIM:600081]; also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. First described in patients who had rickets at a young age despite a history of adequate vitamin D intake. The patients sera had low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activity, and low levels of 25-hydroxyvitamin D.,function:Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D(2) and D(3).,similarity:Belongs to the cytochrome P450 family.,subunit:Homodimer.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
