产品名称
CYP2E1 Rabbit Polyclonal Antibody
别名
CYP2E1; CYP2E; Cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; Cytochrome P450-J
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1571
Human Swissprot No.
P05181
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P05181/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13106
Mouse Swissprot No.
Q05421
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q05421
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25086
Rat Swissprot Link
http://www.uniprot.org/uniprot/P05182
免疫原
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 2E1. AA range:371-420
特异性
CYP2E1 Polyclonal Antibody detects endogenous levels of CYP2E1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008],
组织表达
Brain,Liver,Lung,PCR rescued clones,
细胞定位
Endoplasmic reticulum membrane ; Peripheral membrane protein . Microsome membrane ; Peripheral membrane protein . Mitochondrion inner membrane ; Peripheral membrane protein . Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein. .
信号通路
Arachidonic acid metabolism;Linoleic acid metabolism;Metabolism of xenobiotics by cytochrome P450;Drug metabolism;
功能
catalytic activity:4-nitrophenol + NADPH + O(2) = 4-nitrocatechol + NADP(+) + H(2)O.,cofactor:Heme group.,function:Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.,induction:By ethanol and isoniazid.,online information:CYP2E1 alleles,online information:CYP2E1 entry,similarity:Belongs to the cytochrome P450 family.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
