产品名称
CYP2A6 Rabbit Polyclonal Antibody
别名
CYP2A6; CYP2A3; Cytochrome P450 2A6; 1; 4-cineole 2-exo-monooxygenase; CYPIIA6; Coumarin 7-hydroxylase; Cytochrome P450 IIA3; Cytochrome P450(I)
反应种属
Human,Rat,Mouse,,Chicken
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1548
Human Swissprot No.
P11509
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P11509/entry
免疫原
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 2A6. AA range:1-50
特异性
CYP2A6 Polyclonal Antibody detects endogenous levels of CYP2A6 protein.
稀释度
WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene, CYP2A6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to hydroxylate coumarin, and also metabolizes nicotine, aflatoxin B1, nitrosamines, and some pharmaceuticals. Individuals with certain allelic variants are said to have a poor metabolizer phenotype, meaning they do not efficiently metabolize coumarin or nicotine. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6. [provided by RefSeq, Jul 2008],
细胞定位
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
信号通路
Caffeine metabolism;Retinol metabolism;Drug metabolism;Drug metabolism;
功能
catalytic activity:RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.,cofactor:Heme group.,function:Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase.,induction:By phenobarbital and dexamethasone.,online information:CYP2A6 alleles,online information:CYP2A6 entry,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Liver.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
