产品名称
CYP1A1/2 Rabbit Polyclonal Antibody
别名
CYP1A1; Cytochrome P450 1A1; CYPIA1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; CYP1A2; Cytochrome P450 1A2; CYPIA2; Cytochrome P(3)450; Cytochrome P450 4; Cytochrome P450-P3
蛋白名称
Cytochrome P450 1A1/2
反应种属
Human,Mouse,Rat,Monkey
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1543
Human Swissprot No.
P04798/P05177
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P04798/entry
Mouse Gene ID
13076/13077
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13076
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24296
Rat Swissprot No.
P00185/P04799
Rat Swissprot Link
http://www.uniprot.org/uniprot/P00185
免疫原
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 1A1/2. AA range:71-120
特异性
CYP1A1/2 Polyclonal Antibody detects endogenous levels of CYP1A1/2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016],
组织表达
Lung, lymphocytes and placenta.
细胞定位
Endoplasmic reticulum membrane ; Peripheral membrane protein . Mitochondrion inner membrane ; Peripheral membrane protein . Microsome membrane ; Peripheral membrane protein . Cytoplasm .
信号通路
Steroid hormone biosynthesis;Tryptophan metabolism;Retinol metabolism;Metabolism of xenobiotics by cytochrome P450;
功能
catalytic activity:RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.,cofactor:Heme group.,function:Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.,induction:By 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).,online information:CYP1A1 alleles,online information:CYP1A1 entry,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Lung, lymphocytes and placenta.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
