BD-PT1190-CYP19A1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CYP19A1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT1190

产品名称

CYP19A1 Rabbit Polyclonal Antibody

别名

CYP19A1; ARO1; CYAR; CYP19; Cytochrome P450 19A1; Aromatase; CYPXIX; Cytochrome P-450AROM; Estrogen synthase

类别

常规抗体

基因名称

CYP19A1

蛋白名称

Cytochrome P450 19A1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1588

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1588

Human Swissprot No.

P11511

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P11511/entry

Mouse Gene ID

13075

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13075

Mouse Swissprot No.

P28649

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P28649

Rat Swissprot No.

P22443

Rat Swissprot Link

http://www.uniprot.org/uniprot/P22443

免疫原

The antiserum was produced against synthesized peptide derived from human Cytochrome P450 19A1. AA range:221-270

特异性

CYP19A1 Polyclonal Antibody detects endogenous levels of CYP19A1 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

53kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

cytochrome P450 family 19 subfamily A member 1(CYP19A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],

组织表达

Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

细胞定位

Endoplasmic reticulum membrane ; Multi-pass membrane protein . Microsome membrane ; Multi-pass membrane protein .

信号通路

Steroid hormone biosynthesis;Androgen and estrogen metabolism;

功能

catalytic activity:RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.,cofactor:Heme group.,disease:Defects in CYP19A1 are a cause of familial gynecomastia [MIM:139300]. This is characterized by an estrogen excess due to an increased aromatase activity.,disease:Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.,function:Catalyzes the formation of aromatic C18 estrogens from C19 androgens.,online information:Aromatase entry,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Brain, placenta and gonads.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using Cytochrome P450 19A1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Cytochrome P450 19A1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using Cytochrome P450 19A1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HeLa cells using BCOR antibody.

Western Blot analysis of various cells using CYP19A1 Polyclonal Antibody diluted at 1：1000

Western Blot analysis of 293 cells using CYP19A1 Polyclonal Antibody diluted at 1：1000

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.