产品名称
CYP17A1 Rabbit Polyclonal Antibody
别名
CYP17A1; CYP17; S17AH; Steroid 17-alpha-hydroxylase/17; 20 lyase; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; Steroid 17-alpha-monooxygenase
蛋白名称
Steroid 17-alpha-hydroxylase/17,20 lyase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1586
Human Swissprot No.
P05093
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P05093/entry
Mouse Swissprot No.
P27786
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P27786
免疫原
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 17A1. AA range:221-270
特异性
CYP17A1 Polyclonal Antibody detects endogenous levels of CYP17A1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
cytochrome P450 family 17 subfamily A member 1(CYP17A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008],
组织表达
Brain,Corpus callosum,
细胞定位
Endoplasmic reticulum membrane . Microsome membrane .
信号通路
Steroid hormone biosynthesis;
功能
catalytic activity:A steroid + AH(2) + O(2) = a 17-alpha-hydroxysteroid + A + H(2)O.,cofactor:Heme group.,disease:Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic).,enzyme regulation:Regulated predominantly by intracellular cAMP levels.,function:Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.,online information:The Singapore human mutation and polymorphism database,pathway:Lipid metabolism; steroid biosynthesis.,PTM:Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.,similarity:Belongs to the cytochrome P450 family.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
