产品名称
Cyclin F Rabbit Polyclonal Antibody
别名
CCNF; FBX1; FBXO1; Cyclin-F; F-box only protein 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=899
Human Swissprot No.
P41002
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P41002/entry
Mouse Swissprot No.
P51944
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P51944
免疫原
The antiserum was produced against synthesized peptide derived from human Cyclin F. AA range:737-786
特异性
Cyclin F Polyclonal Antibody detects endogenous levels of Cyclin F protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified. [provided by RefSeq, Jul 2008],
组织表达
Widely expressed, with expression detected in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
细胞定位
Nucleus . Cytoplasm, perinuclear region . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Localization to the centrosome is rare in S phase cells and increases in G2 cells. Localizes to both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110. Localizes to the nucleus in G2 phase. .
功能
developmental stage:G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.,function:Likely to be involved in the control of the cell cycle during S phase and G2.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin AB subfamily.,similarity:Contains 1 F-box domain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
