BD-PT1176-Cyclin E1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Cyclin E1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT1176

产品名称

Cyclin E1 Rabbit Polyclonal Antibody

别名

CCNE1; CCNE; G1/S-specific cyclin-E1

类别

常规抗体

基因名称

CCNE1

蛋白名称

G1/S-specific cyclin-E1

推荐应用

反应种属

Human,Mouse,Rat,Pig

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

898

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=898

Human Swissprot No.

P24864

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P24864/entry

Mouse Gene ID

12447

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12447

Mouse Swissprot No.

Q61457

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q61457

Rat Swissprot No.

P39949

Rat Swissprot Link

http://www.uniprot.org/uniprot/P39949

免疫原

The antiserum was produced against synthesized peptide derived from human Cyclin E1. AA range:91-140

特异性

Cyclin E1 Polyclonal Antibody detects endogenous levels of Cyclin E1 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

49kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in

组织表达

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

细胞定位

Nucleus .

信号通路

Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Oocyte meiosis;p53;Pathways in cancer;Prostate cancer;Small cell lung cancer;

功能

function:Essential for the control of the cell cycle at the G1/S (start) transition.,PTM:Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the cyclin family. Cyclin E subfamily.,subunit:Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with retinoblastoma binding protein 3 and retinoblastoma-like protein 1. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1.,tissue specificity:Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using Cyclin E1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Cyclin E1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from K562 cells, using Cyclin E1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of rat-spleen tissue. 1,Cyclin E1 Polyclonal Antibody（red） was diluted at 1:200（4°,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of various cells using Cyclin E1 Polyclonal Antibody diluted at 1：500

Western Blot analysis of MCF7 cells using Cyclin E1 Polyclonal Antibody diluted at 1：500

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.