产品名称
Cyclin B1 Rabbit Polyclonal Antibody
别名
CCNB1; CCNB; G2/mitotic-specific cyclin-B1
蛋白名称
G2/mitotic-specific cyclin-B1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=891
Human Swissprot No.
P14635
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P14635/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=268697
Mouse Swissprot No.
P24860
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P24860
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25203
Rat Swissprot Link
http://www.uniprot.org/uniprot/P30277
免疫原
The antiserum was produced against synthesized peptide derived from human Cyclin B1. AA range:121-170
特异性
Cyclin B1 Polyclonal Antibody detects endogenous levels of Cyclin B1 protein.
稀释度
WB 1:500-1:2000 IHC 1:50-1:200. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008],
组织表达
Breast adenocarcinoma,Lung,Placenta,
细胞定位
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
功能
developmental stage:Accumulates steadily during G2 and is abruptly destroyed at mitosis.,function:Essential for the control of the cell cycle at the G2/M (mitosis) transition.,PTM:Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin AB subfamily.,subunit:Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
