产品名称
CtIP Rabbit Polyclonal Antibody
别名
RBBP8; CTIP; DNA endonuclease RBBP8; CtBP-interacting protein; CtIP; Retinoblastoma-binding protein 8; RBBP-8; Retinoblastoma-interacting protein and myosin-like; RIM; Sporulation in the absence of SPO11 protein 2 homolog; SAE2
蛋白名称
DNA endonuclease RBBP8
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5932
Human Swissprot No.
Q99708
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q99708/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=225182
Mouse Swissprot No.
Q80YR6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q80YR6
免疫原
The antiserum was produced against synthesized peptide derived from human CTIP. AA range:293-342
特异性
CtIP Polyclonal Antibody detects endogenous levels of CtIP protein.
稀释度
WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008],
组织表达
Expressed in ER-positive breast cancer lines, but tends to be down-regulated ER-negative cells (at protein level).
细胞定位
Nucleus . Chromosome . Associates with sites of DNA damage in S/G2 phase (PubMed:10764811, PubMed:25349192). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604). .
功能
function:May modulate the functions ascribed to BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1.,PTM:Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.,subcellular location:Predominantly nuclear.,subunit:Interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
