产品名称
DDX11 Rabbit Polyclonal Antibody
别名
Probable ATP-dependent RNA helicase DDX11 (EC 3.6.4.13) (CHL1-related protein 1) (hCHLR1) (DEAD/H box protein 11) (Keratinocyte growth factor-regulated gene 2 protein) (KRG-2)
基因名称
DDX11 CHL1 CHLR1 KRG2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.281% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/1663
Human Swissprot No.
Q96FC9
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q96FC9/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/320209
Mouse Swissprot No.
Q6AXC6
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q6AXC6
免疫原
Synthesized peptide derived from human DDX11 AA range: 50-100
特异性
This antibody detects endogenous levels of DDX11 at Human/Mouse
宿主
Polyclonal, Rabbit,IgG
背景介绍
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],
组织表达
Expressed in melanoma cells. Not detected in epidermal melanocytes of normal skin (at protein level) (PubMed:23116066). Highly expressed in spleen, B-cells, thymus, testis, ovary, small intestine and pancreas (PubMed:9013641). Very low expression seen in brain (PubMed:9013641). Expressed in dividing cells and/or cells undergoing high levels of recombination (PubMed:9013641). No expression detected in cells signaled to terminally differentiate (PubMed:9013641). Expressed weakly in keratinocytes (PubMed:8798685).
细胞定位
Nucleus . Nucleus, nucleolus . Cytoplasm, cytoskeleton, spindle pole . Midbody . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . During the early stages of mitosis, localizes to condensed chromatin and is released from the chromatin with progression to metaphase. Also localizes to the spindle poles throughout mitosis and at the midbody at later stages of mitosis (metaphase to telophase) (PubMed:17105772). In interphase, colocalizes with nucleolin in the nucleolus (PubMed:26089203). .; Chromosome . (Microbial infection) Colocalizes with bovine papillomavirus type 1 regulatory protein E2 on mitotic chromosomes at early stages of mitosis. .
功能
function:DNA helicase involved in cellular proliferation. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA.,sequence caution:Translation N-terminally extended.,similarity:Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily.,similarity:Contains 1 helicase ATP-binding domain.,tissue specificity:Highly expressed in spleen, B-cells, thymus, testis, ovary, small intestine, and pancreas. Very low expression seen in the brain. Expressed in dividing cells and/or cells undergoing high levels of recombination. No expression is seen in cells signaled to terminally differentiate. Expressed in keratinocyte growth factor-stimulated cells but not in serum, EGF and IL1-beta-treated keratinocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
