产品名称
cPLA2 Rabbit Polyclonal Antibody
别名
PLA2G4A; CPLA2; PLA2G4; Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA
蛋白名称
Cytosolic phospholipase A2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5321
Human Swissprot No.
P47712
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P47712/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18783
Mouse Swissprot No.
P47713
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P47713
Rat Swissprot Link
http://www.uniprot.org/uniprot/P50393
免疫原
The antiserum was produced against synthesized peptide derived from human c-PLA2. AA range:471-520
特异性
cPLA2 Polyclonal Antibody detects endogenous levels of cPLA2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015],
组织表达
Expressed in various cells and tissues such as macrophages, neutrophils, fibroblasts and lung endothelium. Expressed in platelets (at protein level) (PubMed:25102815).
细胞定位
Cytoplasm . Golgi apparatus membrane . Nucleus envelope. Translocates to intracellular membranes in a calcium-dependent way. .
信号通路
Glycerophospholipid metabolism;Ether lipid metabolism;Arachidonic acid metabolism;Linoleic acid metabolism;alpha-Linolenic acid metabolism;MAPK_ERK_Growth;MAPK_G_Protein;Vascular smooth muscle contraction;VEGF;Fc epsilon RI;Fc gamma R-mediated phagocytosi
功能
catalytic activity:2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate.,catalytic activity:Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.,domain:The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).,enzyme regulation:Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca(2+).,function:Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.,PTM:Activated by phosphorylation at both Ser-505 and Ser-727.,similarity:Contains 1 C2 domain.,similarity:Contains 1 PLA2c domain.,subcellular location:Translocates to membrane vesicles in a calcium-dependent fashion.,subunit:Interacts with HTATIP.,tissue specificity:Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
