产品名称
COT Rabbit Polyclonal Antibody
别名
MAP3K8; COT; ESTF; Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2; TPL-2
蛋白名称
Mitogen-activated protein kinase kinase kinase 8
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1326
Human Swissprot No.
P41279
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P41279/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=26410
Mouse Swissprot No.
Q07174
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q07174
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=116596
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q63562
免疫原
The antiserum was produced against synthesized peptide derived from human COT. AA range:256-305
特异性
COT Polyclonal Antibody detects endogenous levels of COT protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011],
组织表达
Expressed in several normal tissues and human tumor-derived cell lines.
信号通路
SAPK_JNK; Regulation of Actin Dynamics; T_Cell_Receptor; Cell Growth; Stem cell pathway; Toll_Like; MAPK_ERK_Growth;MAPK_G_Protein; B_Cell_Antigen
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,developmental stage:Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle.,function:Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.,PTM:Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Forms a ternary complex with NFKB1 and TNIP2.,tissue specificity:Expressed in several normal tissues and human tumor-derived cell lines.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
