产品名称
COMT Rabbit Polyclonal Antibody
别名
COMT; Catechol O-methyltransferase
蛋白名称
Catechol O-methyltransferase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1312
Human Swissprot No.
P21964
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P21964/entry
Mouse Swissprot No.
O88587
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O88587
免疫原
The antiserum was produced against synthesized peptide derived from human COMT. AA range:61-110
特异性
COMT Polyclonal Antibody detects endogenous levels of COMT protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008],
组织表达
Brain, liver, placenta, lymphocytes and erythrocytes.
细胞定位
[Isoform Soluble]: Cytoplasm .; [Isoform Membrane-bound]: Cell membrane ; Single-pass type II membrane protein ; Extracellular side .
信号通路
Steroid hormone biosynthesis;Tyrosine metabolism;
功能
catalytic activity:S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.,cofactor:Binds 1 magnesium ion per subunit.,function:Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.,mass spectrometry: PubMed:8020475,online information:Catechol-O-methyl transferase entry,polymorphism:Low enzyme activity alleles are associated with genetic susceptibility to alcoholism [MIM:103780].,polymorphism:Two alleles, COMT*1 or COMT*H with Val-158 and COMT*2 or COMT*L with Met-158 are responsible for a three to four-fold difference in enzymatic activity.,PTM:The N-terminus is blocked.,similarity:Belongs to the mammalian catechol-O-methyltransferase family.,tissue specificity:Brain, liver, placenta, lymphocytes and erythrocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
