BD-PT0981-Clusterin Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Clusterin Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0981

产品名称

Clusterin Rabbit Polyclonal Antibody

别名

CLU; APOJ; CLI; KUB1; AAG4; Clusterin; Aging-associated gene 4 protein; Apolipoprotein J; Apo-J; Complement cytolysis inhibitor; CLI; Complement-associated protein SP-40; 40; Ku70-binding protein 1; NA1/NA2; Testosterone-repressed prostate m

类别

常规抗体

基因名称

CLU

蛋白名称

Clusterin

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1191

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1191

Human Swissprot No.

P10909

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P10909/entry

Mouse Gene ID

12759

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12759

Mouse Swissprot No.

Q06890

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q06890

Rat Gene ID

24854

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24854

Rat Swissprot No.

P05371

Rat Swissprot Link

http://www.uniprot.org/uniprot/P05371

免疫原

The antiserum was produced against synthesized peptide derived from human CLU. AA range:400-449

特异性

Clusterin Polyclonal Antibody detects endogenous levels of Clusterin protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

52kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011],

组织表达

Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.

细胞定位

[Isoform 1]: Secreted . Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. .; [Isoform 4]: Cytoplasm . Keeps cytoplasmic localization in stressed and unstressed cell. .; [Isoform 6]: Cytoplasm . Keeps cytoplasmic localization in stressed and unstressed cell. .; Nucleus . Cytoplasm . Mitochondrion membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasm, cytosol . Microsome . Endoplasmic reticulum . Mitochondrion . Mitochondrion membrane . Cytoplasm, perinuclear region . Cytoplasmic vesicle, secretory vesicle, chromaffin granule . Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress (PubMed:17451556). Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins (PubMed:20068069). Detected at the mitochondrion membrane upon induction of apoptosis (PubMed:17689225). Under ER stress, a immaturely glycosylated pre-secreted form retrotranslocates from the endoplasmic reticulum (ER)-Golgi network to the cytoplasm to localize in the mitochondria through HSPA5 interaction (PubMed:22689054). ER stress reduces secretion (PubMed:22689054). Under the stress, minor amounts of non-secreted forms accumulate in cytoplasm (PubMed:24073260, PubMed:22689054, PubMed:17451556). Non-secreted forms emerge mainly from failed translocation, alternative splicing or non-canonical initiation start codon (PubMed:24073260, PubMed:12551933). .

功能

function:Not yet clear. It is known to be expressed in a variety of tissues and it seems to be able to bind to cells, membranes and hydrophobic proteins. It has been associated with programmed cell death (apoptosis).,similarity:Belongs to the clusterin family.,subunit:Antiparallel disulfide-linked heterodimer. Interacts with APOA1, CLUAP1 AND PON1.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using CLUS Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using CLUS Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells and A549 cells, using CLUS Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Clusterin Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.