BD-PT0971-CLK1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CLK1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT0971

产品名称

CLK1 Rabbit Polyclonal Antibody

别名

CLK1; CLK; Dual specificity protein kinase CLK1; CDC-like kinase 1

类别

常规抗体

基因名称

CLK1

蛋白名称

Dual specificity protein kinase CLK1

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1195

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1195

Human Swissprot No.

P49759

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P49759/entry

Mouse Gene ID

12747

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12747

Mouse Swissprot No.

P22518

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P22518

免疫原

The antiserum was produced against synthesized peptide derived from human CLK1. AA range:101-150

特异性

CLK1 Polyclonal Antibody detects endogenous levels of CLK1 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

57kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

CDC like kinase 1(CLK1) Homo sapiens This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009],

组织表达

Endothelial cells.

细胞定位

Nucleus.

功能

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.,PTM:Autophosphorylates on all three types of residues.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with PPIG.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HUVEC cells, using CLK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using CLK1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using CLK1 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western blot analysis of KB lysis using CLK1 antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.